Basics

Training Materials

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SCN Biology and Management (Iowa State University)

Sampling to evaluate the success of an SCN management program

Recommended technique: Collect soil samples and have the SCN population densities determined.

Sampling to determine if SCN is responsible for poor yields

Recommended technique: Collect soil samples and have them tested for the presence of SCN.

Fall SCN Sampling for Next Year's Crop

Recommended technique: Collect soil samples and have them tested for the presence of SCN.

Place the root samples in separate 100 or 150 ml glass beakers. Prepare 600 ml of a water-bleach solution by adding 100 ml household bleach (5% sodium hypochlorite or NaClO) to 500 ml distilled water.

Surface disinfestation of SCN eggs

To prepare the disinfestant solution of 0.5% chlorhexidine diacetate (Hibitane), dissolve 0.5 g into 100 ml sterile distilled water, or dissolve 2.5 g into 500 ml sterile distilled water

To disinfest the eggs:

In-vitro hatching experiments

Construct microsieves from 18-mm and 20-mm test tube caps and 25 micrometer pore nylon mesh.
UV-sterilize all microsieves and hatch trays.

Soybean Cyst Nematode Field Guide

https://store.extension.iastate.edu/Product/Soybean-Cyst-Nematode-Field-Guide